Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids

An electron microscopic method for the mapping of proteins attached to nucleic acids.

An electron microscopic method for demonstrating the presence of and mapping the positions of proteins specifically bound to nucleic acids is described. The nucleic acid-protein complex is treated with dinitrofluorobenzene under conditions such that dinitrophenyl (DNP) groups are attached to nucleophilic groups on the protein, with only a low level of random attachment to the nuclei acid. This ...

NanoDrop microvolume quantitation of nucleic acids.

Biomolecular assays are continually being developed that use progressively smaller amounts of material, often precluding the use of conventional cuvette-based instruments for nucleic acid quantitation for those that can perform microvolume quantitation. The NanoDrop microvolume sample retention system (Thermo Scientific NanoDrop Products) functions by combining fiber optic technology and natura...

Nucleic acids and proteins

Nucleic Acids and Proteins

Fluorometric quantitation of fluorescein-coupled antibodies attached to the cell membrane.

A method was devised which allows the measurement, by direct and indirect fluorescence assay, of the binding of fluorescein-coupled antibodies directed against cell-membrane antigens. The systems used were: (1) species-specific antigens, (2) genetically determined isoantigens, (3) virus-determined, tumor-specific antigens, and (4) membrane-bound immunoglobulin in a Burkitt cell line. Titration ...